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Plus reactions worked differently: they used T4 DNA polymerase, an enzyme with strong 3’ to 5’ exonuclease activity, meaning it can chew back the end of a DNA strand. In the presence of only one dNTP, T4 DNA polymerase would degrade each fragment from its 3’ end until it reached a nucleotide complementary to that dNTP, at which point the exonuclease activity would be inhibited. This ensured that all fragments in a given plus reaction ended with the same nucleotide.

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Horvath blames educational technology (edtech) for these atrophying skill sets, arguing that at the turn of the 21th century and through its first decade and a half, tech companies and advocates pushed a false narrative that the education system was broken, but computers could fix it. Instead, Horvath said, the plan backfired.,这一点在快连下载-Letsvpn下载中也有详细论述

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